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Treatment with 200 M H 2 O 2 successfully induced a robust senescence model in HUVECs ( 2 O 2 -only treatment group (Figure 2G), and the concentration of 0.05 uM of BS had the least effect on the viability of the cells, and -gal staining further confirmed that 0.05 M of BS significantly attenuated hydrogen peroxide-induced HUVEC senescence (Figures 2F,H)

GAPDH served as the internal control

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1983) and subsequently metabolized to MMA and DMA, both of which may exist in the trivalent and pentavalent oxidation states

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